제품/서비스

  • DiaPlexC™ MTCNTM Detection Kit_CE IVD.png
  • DiaPlexC™ MTC/NTM Detection Kit MTC & NTM 단독감염 및 중복감염여부를 정성 검출하는 검사시약
    Multiplex PCR
    CE-IVD

    본 제품은 단 한번의 PCR로 다수의 specific target gene을 검출할 수 있는 multiplex PCR 기술을 이용하여 결핵균 (MTC) 5종과 비결핵균 (NTM) 20종의 co-infection 및 single infection을 검사하는 키트 입니다. 제품 내의 오염요인을 줄이기 위해 DNA free Taq을 사용하였으며, UDG system을 적용하여 PCR product에 의한 carryover contamination을 방지, 오염에 의한 위양성의 가능성을 줄였습니다.
    사용의 편리함을 위해 enzyme과 dNTP, buffer, bromophenol blue 등을 섞은 Smart mix 형태로 제조하여, 사용자가 template DNA와 primer mixture만 첨가하여 간편하게 PCR을 수행할 수 있습니다. 또한 positive control인 Control Template가 제공됩니다.

제품사양
제품사양
DiaPlexC™ MTC/NTM Detection Kit
Detection target MTC (2 species), NTM (10 species)
Registration CE-IVD
Detection technology Conventional (End-point) Multiplex PCR
Specimen type 객담(Sputum), 비인두 swab(Nasopharyngeal swab), 인두 비강 흡입(Nasopharyngeal aspirate),
기관지폐포 세척액(Bronchoalveolar lavage, BAL)
Analytical sensitivity 102 - 105 copies
Compatible instruments* ABI Veriti thermal Cycler (Applied Biosystems) recommended
PCR running time ~ 1 hr 30 min
제품특징
제품특징

- HotStart PCR system : Ultra hugh specific and sensitive result
- DnaFree™ system : No host genomic DNA contamination ( DiaPlexC ™)
- UDG system : No carryover contamination
- Multiplex PCR system : Multiple targets in a single reaction
- Reliable system : Automatic PCR control
- Positive control included
- DNA extraction solution included (Cat. No. SQD21-K100)
- Easy-to-use master mix
- CE certification

실험데이터
1-1.JPG
Lane Interpretation (detection)
1 NTM
2 MTC
3 MTC, NTM (Co-infection)
4 Negative (or NTC)
5 Required re-experiment
인용&논문
  1. Korean J Clin microbial, Seong Deok Lee, Hye Young Lee, Hyun Chul Kim, Soo Young Kim,

    Mycobacterium tuberculosis and Non-Tuberculous Mycobacteria by PCR Assay.

  2. Ryan KJ, Ray CG (Editors) (2004).

    Sherris Medical Microbiology (4th ed.). McGraw-Hill. Clinical Characteristics of Non-Tuberculous Mycobacterial Pulmonary Disease in a National Tuberculosis Hospital.

  3. Sun-Pil Choi, M.D., Bong-Keun Lee M.D.1, Jin-Hong Min, M.D., Jin-Hee Kim, M.D.

    pathogenic Classification and Clinical Characteristics of Non-Tuberculous Mycobacterial Pulmonary Disease in National Tuberculosis Hospital

주문정보 (Ordering information)
Technology Cat. No. Product Contents
Conventional (End-point) PCR SMD21-K020 (20 reaction) DiaPlexC ™ MTC/NTM Detection Kit 2X Multiplex PCR Smart mix (with UDG) (MTC/NTM)
Primer Mixture (MTC/NTM)
Standard Marker (MTC/NTM)
Control Template (MTC/NTM)
Nuclease free Water
SMD21-K100 (100 reaction)